The allelopathic effect of biomass of Ganoderma species (9 strains of 6 species) on Cucumis sativus seeds germination and growth of root and shoot was determined by using a modified sandwich method. It was demonstrated that the biomass of some investigated strains shows allelopathic activity. Mycelia of Ganoderma resinaceum 2477 and Ganoderma lucidum 1904 inhibits the growth of Cucumis sativus by 61%, mycelia of Ganoderma sinense 2516, Ganoderma tsugae 2024 and Ganoderma carnosum 2502 increased the average length of the shoots by 35.7- 42.9% compared to the control group. These results suggest that Ganoderma species have a regulatory effect on cucumber sprouting growth.