The cultural and morphological characteristics of three wood-inhabiting Xylaria Hill ex Schrank species (23 strains) from the IBK Mushroom Culture Collection were studied. Data on radial mycelial growth, detailed descriptions and illustrations of colonies on two different agar nutrient media were provided. For X. polymorpha (Pers.) Grev., X. longipes Nitschke and X. hypoxylon (L.) Grev. differences in mycelial colony characteristics (textures, colors, concentric zones, margins, formation of stromata and characteristics of reverse) were observed.