ISSN 2617-8001 (Print), ISSN 2664-5297 (Online)
COMPOSITION AND CONTENT OF PHENOLIC ACIDS IN FRUIT JUICE AND FLOWERS OF PUNICA GRANATUM L.
Aydan M. Zeynalova, Eldar N. Novruzov

Pomegranate (Punica granatum L.) is an important source of bioactive compounds. These compounds are primarily phenolic or polyphenolic, flavonoids (catechins and isoflavones) and related compounds (phenolic acids and chalcones). The purpose of this study was to assess the quantitative and qualitative determination of phenolic acids in fruit juice and flowers of wild-growing pomegranate. For the first time the component composition and the content of phenolic acids in the juice of fruits and flowers of wild-type pomegranate growing on the territory of Galalti, Siyazan region of the Azerbaijan Republic was assessed by using high-performance liquid chromatography (HPLC). Chromatographic analysis was performed using Varian LC/MS (US Ser. No. 05755). Our results show that the samples of flowers and juice differ in both the qualitative and quantitative content of phenolic acids. The composition of the extract of flowers revealed the presence of caffeic, chlorogenic, cinnamic, ferulic, gallic, p-coumaric and sinapic acids. The same compounds were found in the fruit juice, with the exception of gallic acid. The main components in flowers were sinapic and ferulic acids, which constitute 98.5% of the total amount of phenolic acids, wheres in juice a prevalence of chlorogenic (43.4%) and sinapic (37.1%) acids were found. Most of the identified phenolic acids are well known to exhibit pharmaceutical properties, therefore it suggests that flowers and juice of wild-growing pomegranate might be exploit as a potential source of pharmaceutically active compounds and natural biopesticides.  

Keywords: bioactive compounds, high performance liquid chromatography, identification and quantitative determination
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