The genus Ferula L. (Apiaceae) has 130 species, widespread in the Caucasus, Central Asia, Siberia, China (Xinjiang), Afghanistan, Iran and North Africa. There are eight species of this genus in the Republic of Azerbaijan. The main part of the chemical substances of the plants of genus Ferula species are sesquiterpene coumarins, lactones, alcohol esters and essential oils. For the first time, a crystalline substance with the composition C₂₄H₃₂O₅ with a melting point of 157-159°C was isolated from an ethanol extract of Ferula persica roots collected in the phase of full ripening of seeds using column chromatography. Based on spectral (UV)-ultraviolet, (IR)-infrared, (NMR)-nuclear magnetic resonance PMR and ¹³C NMR, DEPT and two-dimensional experimental methods COSY, HSQC, HMBC and physical indicators (polarometry, melting point) data, it has been proven that this substance has a structure identical to mogoltavidin. To determine the configuration of a substance in the PMR spectrum, were used which is a classical method the chemical shift and the value of the spin-spin interaction constant. The purpose of the current study was to identify the coumarin content of F. persica roots. Obtained results in the future can be assessed for identifying chemotoxonomic position of the species within the genus.